考虑径流非一致性的水库分期旱限水位优化研究

【目的】分析变化环境下水文序列的非一致性,为龙羊峡水库分期旱限水位优化调度模型的建立提供参考。【方法】采用Mann-Kendall检验法、有序聚类分析法和水文序列振动中心重构法,对龙羊峡水库年径流序列进行一致性分析及修正,然后利用水库旱限水位分级和Fisher最优分割法,对径流序列修正前后不同干旱等级下的水库分期旱限水位进行确定,最后通过耦合分期旱限水位建立以缺水指数最小为目标函数的龙羊峡水库优化调度模型,对径流序列修正前后两种情况下的分期旱限水位进行对比研究。【结果】一致性检验发现,龙羊峡水库径流序列具有明显下降的趋势,并在1989年发生突变。经一致性修正后计算龙羊峡水库7-10月、11-12月、1-4月、5-6月4个分期的旱限水位,在轻旱条件下分别为2 530.00,2 534.91,2 539.10和2 535.13 m,中旱条件下分别为2 530.00,2 535.44,2 539.80和2 536.70 m,重旱条件下分别为2 530.00,2 535.87,2 540.35和2 537.89 m;特旱条件下分别为2 530.00,2 536.06,2 540.58和2 538.12 m。采用不同调度模型对龙羊峡水库进行优化调度,结果显示修正后的调度效果较好,其总缺水量、缺水指数相比修正前分别降低5.37%和6.03%,其中枯水时段平均缺水量降低了10.71%,缺水程度改善明显。【结论】非一致性径流对龙羊峡水库旱限水位的确定有一定影响,一致性修正后的径流使水库旱限水位的确定更加可靠,水库的抗旱能力得以提升,可为水库供水、抗旱应急管理决策提供参考。     

Effect of group size and regrouping on physiological stress and behavior of dairy calves

Mixing or regrouping of calves from different pens is a common animal management practice on the farm, which frequently occurs after weaning and has a negative effect on calve welfare.  Social integration before regrouping may relieve stresses, but more evidences are needed to verify this hypothesis.  The present study aimed to investigate acute physiological and behavioral variations of individually- or group-housed calves after being introduced into a mixed group.  A total of 132 postnatal calves were randomly divided into groups of 1, 3, 6 and 12 animals (S, G3, G6, and G12; 6 replicates in each group) until 59 days of age.  At 60 days of age, every two replicates from different groups (S, G3, G6 and G12) were introduced in a larger pen which containing 44 of the aboved experimental calves.  Before and after regrouping, physiological parameters of stress, including heart rate (HR), saliva cortisol (S-CORT), saliva secretory immunoglobulin A (SIgA), interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) levels, and behavioral responses were recorded.  After regrouping, HR and S-CORT increased immediately (P<0.05), and higher (P<0.05) levels of such molecules were found in S calves compared to those in group-housed calves.  Levels of SIgA and IL-2 were decreased (P<0.05), and the lowest (P<0.05) IL-2 values were found in S calves compared to those in group-housed calves.  In addition, the introduced calves displayed a distinct behavior, including altered active and rest time, which was associated with negative emotions triggered by the novel surroundings.  Allogrooming, play, exploration behaviors and lying time were increased significantly (P<0.05) in group-housed calves than those in S calves.  Conversely, self-grooming, aggressive behaviors, standing and walking time were increased (P<0.05) in S calves than those in group-housed calves.  These findings suggest that individually-housed calves may be more susceptible to stressors arising from regrouping than group-housed calves, which consequently negatively affected behavioral and neuroendocrine responses.  Furthermore, moving calves with previous social experience may help mitigate regrouping stress.     

Advancing approach and toolbox in optimization of chloroplast genetic transformation technology

Chloroplast is a discrete, highly structured, and semi-autonomous cellular organelle. The small genome of chloroplast makes it an up-and-coming platform for synthetic biology. As a special means of synthetic biology, chloroplast genetic engineering shows excellent potential in reconstructing various sophisticated metabolic pathways within the plants for specific purposes, such as improving crop photosynthetic capacity, enhancing plant stress resistance, and synthesizing new drugs and vaccines. However, many plant species exhibit limited efficiency or inability in chloroplast genetic transformation. Hence, new transformation technologies and tools are being constantly developed. In order to further expand and facilitate the application of chloroplast genetic engineering, this review summarizes the new technologies in chloroplast genetic transformation in recent years and discusses the choice of appropriate synthetic biological elements for the construction of efficient chloroplast transformation vectors.     

三生空间视角下基于最小阻力模型的农村居民点布局优化——以吉林省辉南县为例

为了科学划分农村居民点类型,对不同类型农村居民点提出相应的整治措施,本研究以吉林省辉南县为例,构建生产-生活-生态空间评价指标体系,采用最小阻力模型和K-均值聚类分析划分村庄组团类型,依据改进的空间引力模型和加权Voronoi图结果,进一步在村庄组团内部划分出不同的农村居民点类型。结果表明,研究区域“三生”空间综合阻力值分布差异性特征显著,总体呈东高西低趋势,按照综合阻力值大小分为最小、较小、中度、较大、最大5个阻力区。基于综合阻力分区结果与聚类结果划分出4种村庄组团类型：集聚提升类村庄47个,总面积为1.243×10⁷ m²;城郊融合类村庄35个,总面积为7.169×10⁶ m²;特色保护类村庄41个,总面积为1.252×10⁷ m²;稳定改善类村庄18个,总面积为4.201×10⁶ m²。根据村庄组团内部相对引力值关系和每个农村居民点实际的功能辐射范围,进一步在村庄组团内部划分出重点建设型、一般建设型、迁移合并型3种农村居民点类型,并在此基础上对农村居民点布局优化提出相应的整治策略。研究表明,生产-生活-生态空间评价指标体系可以较好地反映外界因素组合对辉南县农村居民点布局的影响,基于最小阻力模型等多种空间分析方法划分村庄组团及其内部组合特征,能够科学划分农村居民点类型,从而对不同农村居民点类型提出相应的治理措施,制定符合当地发展的策略。     

Differential structure and physiological response to canker challenge between ‘Meiwa’ kumquat and ‘Newhall’ navel orange with contrasting resistance

Asiatic citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most detrimental diseases affecting citrus production. ‘Meiwa’ kumquat (Fortunella crassifolia) and ‘Newhall’ navel orange (Citrus sinensis) are two genotypes with contrasting Xcc resistance. In the current study, efforts were made to perform pairwise comparison between them in terms of structural difference and biochemical response to Xcc attack. Pinprick inoculation demonstrated that ‘Meiwa’ had lower disease incidence rate and smaller lesion size, concurrent with significantly less bacterial number at the inoculation sites relative to ‘Newhall’. Microscopic observation demonstrated that stomatal density and size of ‘Meiwa’ were significantly smaller than ‘Newhall’. Stomatal apertures of ‘Meiwa’ were smaller than ‘Newhall’ measured at 2 h after their leaves were immersed in Xcc suspension, consistent with the bacteria number in the internal tissues counted at 4 d. Epicuticular wax content of ‘Meiwa’ was significantly higher than that of ‘Newhall’, while no difference in palisade and spongy tissue was observed between them. Enzymatic activity assay showed that after Xcc inoculation, ‘Meiwa’ exhibited significantly higher activities of catalase (CAT), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) in comparison with ‘Newhall’ at most of the sampling time points. Our data showed that the two genotypes with contrasting Xcc resistance exhibited clear difference in the structure and physiological response, which may provide information to facilitate our understanding of Xcc resistance.     

基于智能算法的猪饲料配方优化数学模型

在多种原料配比过程中，营养指标因素复杂的实际情况及控制成本保证收益的现实需求，文章结合智能算法背景，进行猪饲料配方优化设计，建立猪饲料配方模型，采用智能算法求解饲料配方的过程，对于生猪饲料配方进行建模，同时优化出饲料配方。结果表明，智能算法的猪饲料配方优化能够解决多种原料配比以及多个营养指标复杂因素，实现快速计算和生产处理，对企业生产者、销售经营者、实际消费者具有十分重大的价值与现实意义。 [关键词]智能算法|猪饲料|配方优化|数学模型     

高能湿法球磨制备鱼胶原肽螯合钙及其理化特征

为了拓展胶原肽螯合钙的制备工艺,以鱼胶原肽和CaCl2为原料,以高能湿法球磨的方法制备了鱼胶原肽螯合钙,并采用FT-IR红外图谱、X-衍射、EDS能谱等技术手段对其理化特性和结构进行分析。结果显示,随着球磨时间从15 min延长到240 min,鱼胶原肽的平均粒径逐渐降低,ZETA电位绝对值、分子量和pH值逐渐增加,鱼胶原肽对钙的螯合率逐渐从74.21%增加到85.42%。鱼胶原肽螯合钙与鱼胶原肽的主体化学结构相似,但是球磨后鱼胶原肽的-COO-1 VAS吸收峰(1 643.89 cm~(-1))向短波数方向移动到1 540～1 555 cm~(-1),而-NH2的特征吸收峰(3 313.9 cm~(-1))向长波数方向移动到3 337.12～3 380.59 cm~(-1)。球磨后鱼胶原在2θ=20°处的特征宽吸收峰消失,而在2θ=26、28、48和58°处出现多个尖峰和弥散峰,表明其从无定形结构向兼具结晶和无定形结构转变。鱼胶原肽螯合钙中钙元素比例逐渐增加,证实鱼胶原肽对钙的螯合率增加。研究表明,高能湿法球磨可应用于鱼胶原肽螯合钙的制备,该方法具有加工工艺简单、无污染和螯合率高等优点。     

生鲜肉类食品供应链碳排放测算及动态优化研究

以生鲜肉类食品供应链作为研究对象,基于生命周期理论界定生鲜肉类食品供应链碳排放的系统边界,并结合系统动力学方法建立生鲜肉类食品供应链的系统动力学模型,探讨肉类食品供应链的碳排放问题。研究结果表明:1)1t生鲜肉在其生命周期内产生的碳排放为166.97kg,其中生产、运输、仓储和销售环节中碳排放分别占总供应链的28.68%、52.37%、8.90%和10.04%,运输环节对全供应链的碳排放贡献率最高。2)动态优化研究发现,运输环节改变长途运输方式、生产环节废弃物回收利用及优化生产设备效率、销售环节降低设备功率取得的碳排放优化效果最佳,在2018年供应链碳排放分别降低了41.10%、4.70%、1.61%和1.25%。3)目前优化运输能源结构、开发可再生的清洁能源已成为大势所趋,且模拟结果表明,运输中燃烧生物柴油可降低供应链碳排放的13.10%,减排效果较为显著。4)在生鲜肉预冷过程中降低制冷系统能耗、在冷藏及销售过程中提高设备空间利用率可分别使供应链年碳排放降低2 100、1 600t,虽实施效果较小,但该措施便于操作,是降低供应链碳排放的有效途径。     

基于SOA的水轮机调速系统PID参数优化

针对现有优化方法复杂、易陷入局部最优等问题,为水轮机调速系统提出了一种基于人群搜索算法的比例-积分-微分控制参数优化方法.为验证该算法的可行性,建立了水轮机调速系统非线性模型,选取水轮发电机组转速偏差的积分时间绝对误差指标作为目标函数进行优化.为验证优化结果的有效性,将人群搜索算法的控制效果与参考文献中粒子群算法的控制效果进行了对比分析.仿真结果表明,在5%频率扰动下,人群搜索算法自第29次迭代起已收敛,经其优化的系统能在8秒内趋于稳定,此时系统的超调量为1.6%;在10%负荷扰动下,人群搜索算法自第25次迭代起已收敛,其优化效果与粒子群算法优化效果基本相同,两者均在10秒内让系统趋于稳定,但人群搜索算法优化的积分时间绝对误差指标比粒子群算法优化的积分时间绝对误差指标小,表明人群搜索算法具有更好的搜索功能,在一定程度上改善了孤网运行条件下机组的动态性能.     

鄱阳湖区反嘴鹬种群数量动态与空间分布格局

2002—2015年,采用同步调查法对鄱阳湖区反嘴鹬(Recurvirostra avosetta)越冬种群数量动态与空间分布格局进行了监测。结果表明:鄱阳湖区分布有稳定的反嘴鹬越冬种群,年平均数量为(16684±14653)只,2004年、2005年、2013年种群数量均超过目前其全球数量的10%,2004年冬季鄱阳湖区越冬反嘴鹬数量达到最大值,为47520只。鄱阳湖区共有70个湖泊记录到反嘴鹬,其中反嘴鹬种群数量达到全球1%以上的湖泊有20个。每年冬季鄱阳湖区反嘴鹬集中分布在常湖、蚌湖、花庙湖和下坝湖,而反嘴鹬利用频次最高的是大湖池,每年反嘴鹬的越冬数量为(1819±1593)只。各湖泊中反嘴鹬的年平均数量与利用频次之间不存在显著相关性。自然保护区涵盖了反嘴鹬在鄱阳湖越冬的主要湖泊,分布在保护区内的反嘴鹬种群占鄱阳湖区越冬总数量的(75.3±26.0)%,但仍有很多重要的湖泊没有被纳入到保护区范围。反嘴鹬对保护区内湖泊的利用频次与保护区外湖泊的利用频次不存在显著性差异,保护区内反嘴鹬的种群数量显著大于保护区外。